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KMID : 0379520070230020123
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한국독성학회지
2007 Volume.23 No. 2 p.123 ~ p.126
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Inhibition of the Induction of Nitric Oxide Synthase by Kobusin
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Kim Sang-Kyum
Pokharel Yuba-Rai
Kim Ok
Woo Eun-Rhan
Kang Keon-Wook
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Abstract
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We isolated a lignan, kobusin from Geranium thunbergii and studied its effect on the expression of inducible nitric oxide synthase (iNOS) gene in a monocyte/macrophage cell line, RAW264.7 cells. Kobusin inhibited lipopolysaccharide (LPS)-stimulated NO production and the expression of iNOS in a concentration-dependent manner. To identify the mechanistic basis for its inhibition of iNOS induction, we examined the effect of kobusin on both the luciferase reporter activity using NF-{kappa}B minimal promoter and the nuclear translocation of p65. Kobusin suppressed the reporter gene activity and the LPS-induced movement of p65 in to nucleus. NF-{kappa}B activation is controlled by the phosphorylation and subsequent degradation of I-{kappa}B{alpha}, and in the present study, we found that I-{kappa}B{alpha} phosphorylation was also inhibited by kobusin. Our findings indicate that kobusin may provide a developmental basis for an agent against inflammatory diseases.
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KEYWORD
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Geranium thunbergii, iNOS, Kobusin, NF-{kappa}B, Nitric oxide
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